Trichome Lengths of the Heterocystous N\u3csub\u3e2\u3c/sub\u3e-Fixing Cyanobacteria in the Tropical Marginal Seas of the Western North Pacific

Calothrix rhizosoleniae and Richelia intracellularis are heterocystous cyanobacteria found in the tropical oceans. C. rhizosoleniae commonly live epiphytically on diatom genera Chaetoceros (C-C) and Bacteriastrum (B-C) while R. intracellularis live endosymbiotically within Rhizosolenia (R-R), Guinardia (G-R), and Hemiaulus (H-R); although, they occasionally live freely (FL-C and FL-R). Both species have much shorter trichomes than the other marine filamentous cyanobacteria such as Trichodesmium spp. and Anabaena gerdii. We investigated the trichome lengths of C. rhizosoleniae and R. intracellularis in the South China Sea (SCS) and the Philippine Sea (PS) between 2006 and 2014. On average, H-R had the shortest trichome lengths (3.5 cells/trichome), followed by B-C and C-C (4.9–5.2 cells/trichome) and FL-C (5.9 cells/trichome), and R-R, G-R, and FL-R had the longest trichome lengths (7.4–8.3 cells/trichome). Field results showed the trichome lengths of C-C and B-C did not vary seasonally or regionally. However, FL-C and H-R from the SCS and during the cool season had longer trichomes, where/when the ambient nutrient concentrations were higher. R-R, G-R, and FL-R also showed regional and seasonal variations in trichome length. Ultrastructural analysis found no gas vesicles within the C. rhizosoleniae cells to assist in buoyancy regulation. Results suggest that the trichome lengths of C. rhizosoleniae and R. intracellularis might be regulated by their diatom hosts’ symbiotic styles and by ambient nutrients. Short trichome length might help C. rhizosoleniae and R. intracellularis to stay in the euphotic zone regardless as to whether they are free-living or symbiotic

The Impact of Eddies on Nutrient Supply, Diatom Biomass and Carbon Export in the Northern South China Sea

We have investigated the effect of eddies (cold and warm eddies, CEs and WEs) on the nutrient supply to the euphotic zone and the organic carbon export from the euphotic zone to deeper parts of the water column in the northern South China Sea. Besides basic hydrographic and biogeochemical parameters, the flux of particulate organic carbon (POC), a critical index of the strength of the oceanic biological pump, was also measured at several locations within two CEs and one WE using floating sediment traps deployed below the euphotic zone. The POC flux associated with the CEs (85 ± 55 mg-C m −2 d −1) was significantly higher than that associated with the WE (20 ± 7 mg-C m −2 d −1). This was related to differences in the density structure of the water column between the two types of eddies. Within the core of the WE, downwelling created intense stratification which hindered the upward mixing of nutrients and favored the growth of small phytoplankton species. Near the periphery of the WE, nutrient replenishment from below did take place, but only to a limited extent. By far the strongest upwelling was associated with the CEs, bringing nutrients into the lower portion (∼50 m) of the euphotic zone and fueling the growth of larger-cell phytoplankton such as centric diatoms (e.g., Chaetoceros, Coscinodiscus) and dinoflagellates (e.g., Ceratium). A significant finding that emerged from all the results was the positive relationship between the phytoplankton carbon content in the subsurface layer (where the chlorophyll a maximum occurs) and the POC flux to the deep sea